![]() ![]() Here, we present the latest version of our iCLIP protocol, discussing critical aspects and recent modifications. We recently described individual-nucleotide resolution CLIP (iCLIP) - an approach that both increases data complexity and allows binding site detection at single-nucleotide resolution. This, in turn, complicates the identification of individual binding sites. UV cross-linking and immunoprecipitation (CLIP) and individual-nucleotide resolution CLIP (iCLIP) are methods to study protein-RNA interactions in untreated cells and tissues. UV cross-linking and immunoprecipitation (CLIP) and individual-nucleotide resolution CLIP (iCLIP) are methods to study protein-RNA interactions in untreated. However, data complexity is often limited and the resolution of the resulting maps is confined to approximately 30 nucleotides. UV cross-linking and immunoprecipitation (CLIP) is a state-of-the-art technique for generating such maps on a genome-wide scale. Therefore, understanding post-transcriptional regulation requires precise and comprehensive binding site maps for RBPs. Here, we present the latest version of our iCLIP protocol, discussing critical aspects and recent modifications.ĪB - Post-transcriptional regulation of gene expression is controlled by the unique composition and spatial arrangement of RNA-binding proteins (RBPs) on individual transcripts. Hansen, Heidi Theil Rasmussen, Simon Horskjr Adolph, Sidsel Kramshj. We recently described individual-nucleotide resolution CLIP (iCLIP) - an approach that both increases data complexity and allows binding site detection at single-nucleotide resolution. Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation. This, in turn, complicates the identification of individual binding sites. However, data complexity is often limited and the resolution of the resulting maps is confined to approximately 30 nucleotides. To systematically identify the RNAs that interact with NF90, we carried out iCLIP (individual-nucleotide resolution UV crosslinking and immunoprecipitation) analysis in the human embryonic. N2 - Post-transcriptional regulation of gene expression is controlled by the unique composition and spatial arrangement of RNA-binding proteins (RBPs) on individual transcripts. T1 - Analysis of Protein-RNA Interactions with Single-Nucleotide Resolution Using iCLIP and Next-Generation Sequencing ![]()
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